教育厅研究生教育创新计划项目（粤教研函[2013]18号）
[通信作者] *成金乐，教授，研究方向为创新中药研究与开发，Tel：（0760）85312768，E-mail：gdcjl9@126.com
[作者简介] 杨泽锐，硕士研究生，研究方向为现代中药新药研究，E-mail：1264041164@qq.com
Preliminary study on effect of Rhodiolae Crenulatae Radix et Rhizoma
cell wall-broken decoction pieces on intestinal flora of mice
YANG Ze-rui1， 2， 3， ZENG Gui-mei1， 2， 3， PENG Li-hua2， 3， ZHANG Miao-miao4， CHENG Jin-le2， 3*， ZHAN Ruo-ting1
（1. Chinese Medicinal Resource Science and Engineering Research Center of Guangzhou University of Chinese
Medicine， Key Laboratory of Chinese Medicine Resources from Lingnan Under Ministry of Education， Guangzhou 510006， China;
2. Key Laboratory of Cell Wall-Broken Decoction Pieces Technology and Application of State Administration of Traditional
Chinese Medicine， Zhongshan 528437， China;
3. Cell Wall-Broken Powder Engineering Technology Research and Development Center of Guangdong Province， Zhongshan 528437， China;
4. College of Fundamental Medicine， Guangzhou University of Chinese Medicine， Guangzhou 510006， China）
[Abstract] This study aims to analyze and compare the effect of cell wall-broken decoction pieces， conventional decoction pieces and conventional powder of Rhodiolae Crenulatae Radix et Rhizoma on the intestinal flora of normal mice. The conventional bacterial culture and PCR-DGGE （polymerase chain reaction-denaturing gradient gel electrophoresis） were adopted for the mice after the oral administration for 14 days. According to the bacterial culture results， the 1/8 dose cell wall-broken decoction pieces group showed fewer Enterococcus and Escherichia coli bacillus but more Lactobacillus and Bifidobacterium than the conventional decoction pieces group and the traditional powder group （P<0.05）. Meanwhile， on the basis of the PCR-DGGE results， the 1/8 dose cell wall-broken decoction pieces group revealed the highest Shannon-Wiener index （H） and species richness （S） among the seven groups， with extremely significant differences compared with the normal group （P<0.01）， significant differences compared with the conventional decoction pieces group and the conventional powder group （P<0.05） and a high intra-group similarity. In conclusion， the long-term intake of 1/8 dose Rhodiolae Crenulatae Radix et Rhizoma cell wall-broken decoction pieces showed a certain effect in regulating intestinal tract by promoting the growth of Lactobacillus and Bifidobacterium. Furthermore， the intestinal flora community will become more stable.