对照高出18.85%、40.67%、76.28%和37.50%，接种Q67的处理各项指标分别比对照高出27.47%、42.81%、96.05%和42.97%，差异显著；接种Q6-2和Q67后，叶绿素含量分别高出对照43.27%和51.46%，根系活力则分别高出5.41%和7.05%，差异显著。经鉴定，Q6-2和Q67均为地衣芽孢杆菌，GenBank登录号分别为KP686129和KP686133。2株细菌有效促进了芡实幼苗生长发育水平，在微生物肥料的生产中具有广阔的应用前景。
关键词：芡实；植物根际促生菌；植物激素；促生；鉴定
中图分类号：S154.38+1 文献标识号：A 文章编号：1001-4942（2015）08-0053-06
Abstract This study aims to provide excellent bacterial strain resources for the production of Euryale ferox bio-fertilizer. Two strains with relatively higher performance named Q6-2 and Q67 were screened from the rhizosphere soil of Euryale ferox using the methods of remaining green and radish cotyledon bioassay； their production capability of plant hormones and organic acids were quantitatively analyzed by HPLC； their promotion effects on the growth of Euryale ferox at seedling stage were investigated by pot experiments； also， they were identified by physiological and biochemical experiments and 16S rDNA sequence analysis. The results indicated that the two strains were both able to produce zeatin， kinetin and small amount of organic acid. Q6-2 was also able to produce indoleacetic acid （IAA）. After inoculated by Q6-2， the growth parameters of Euryale ferox seedlings， such as stem length， number of leaves， maximum leaf area and biomass （dry weight）， significantly increased by 18.85%， 40.67%， 76.28% and 37.50% respectively compared to the control； while inoculated by Q67， the above mentioned indexes significantly increased by 27.47%， 42.81%， 96.05% and 42.97% respectively. After inoculated by Q6-2 and Q67， the chlorophyll content increased by 43.27% and 51.46% respectively， and the root activity increased by 5.41% and 7.05% respectively， showing significant differences compared to the control. Q6-2 and Q67 were both identified as Bacillus licheniformis， whose GenBank accession number were KP686129 and KP686133 respectively. In conclusion， the two strains were able to promote the growth and development of Euryale ferox seedlings efficiently， showing great application prospect in the production of microbial fertilizer.
Key words Euryale ferox； Plant growth-promoting rhizobacteria； Plant hormones； Growth promoting； Identification